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Reference #:
2004-244
Inventors/Contributors
Ravinder Jit Singh, Ph.D., Robert L. Taylor, Stefan Grebe, M.D., Ph.D
Description
Deuterated stable isotope (d3-25-hydroxyvitamin D) is added to a 0.2-mL plasma sample as internal standard. 25-Hydroxyvitamin D2, D3, and the internal standard are extracted using acetonitrile precipitation. The extracts are then further purified online and analyzed by LC-MS/MS using multiple reaction monitoring. 25-OH-VitD2 and 25-OH-VitD3 are quantified and reported individually and as a sum with a clinical reference range attached to the sum.
Patent Status
Pending |
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Contact
Barbara A. Gamez Sims, Licensing Manager
gamezsims.barbara@mayo.edu
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Mayo Foundation for Medical Education and Research
Office of Technology Commercialization
Centerplace 4
200 First Street SW
Rochester, MN 55905
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Phone: (507) 284-8171
Fax: (507) 284-5410
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