Detection of Cytochrome P450 (CYP) 2D7-CYP2D6 Recombinant Genes

Reference #:

2007-166

Inventors/Contributors

John L. Black, III, M.D., David A. Mrazek, M.D., Dennis J. O'Kane, Ph.D.

Description

This invention describes a method that allows for the detection of the CYP2D7-CYP2D6 recombinant gene. This gene encodes a protein which produces an enzyme incapable of metabolizing drugs. This is in distinct from the normal CYP2D6 gene which is able to metabolize some drugs such as some antidepressants and tamoxifen. Therefore, the technique is useful for pharmacogenomic testing for patients receiving some antidepressants or tamoxifen which are metabolized by CYP2D6. The technique is as follows: First a polymerase chain reaction (PCR) is done to produce an amplicon unique to the CYP2D7-CyP2D6 recombinant gene (e.g. CYP2D6*13 and *16). This is followed by an allele specific primer extension assay using Tag-It Mutation Detection Kit P450-2D6.

Patent Status

None