Dominant Negative SINA/SIAH Cell Lines and Transgenic Mouse Lines

Reference #:

2007-240

Inventors/Contributors

Amy Hong Tang Ph.D.

Description

A number of stable human pancreatic cancer cell lines (MiaPaCa and Panc-1) have been generated that expressed SIAH-1-DN and SIAH-2-DN proteins are different levels at the Mayo Clinic Rochester. These SIAH-DN-expressing cancer cells have normal cell cycle and cell proliferation profiles under tissue culture condition when everything the cells need are provided for in the medium. However, excitingly, these SIAH-DN-expressing cells do not form tumors when implant subcuteneously in the athymic nude mice while, in contrast, their control cells formed large tumors in athymic nude mice. We have frozen down these cells and aliquoted them. In addition, we have the following four transgenic mouse lines: (1) CMV promoter-driven-SIAH-1-DN, (2) CMV promoter-driven-5' FLAG tagged SIAH-1-DN, (3) Pdx-1 promoter-driven-5' FLAG tagged SIAH-1-DN, and (4) Pdx-1 promoter-driven-5' FLAG tagged SIAH-1-DN. We have successfully bred and amplified these transgenic mice. Currently, we are using these SIAH-DN-expressing transgenic mice in attempt to block tumorigenesis of the oncogenic K-RAS (+)-mediated lung cancer models (Johnson et al., Nature 410, 1111-1116, 2001) and oncogenic K-RAS (+)-mediated pancreatic cancer models (Hingorani et al., Cancer Cell 4, 437-450, 2003; Aguirre et al., Genes & Development, 17;3112-3126, 2003; Hingorani et al., Cancer Cell 7, 469-483, 2005).

Patent Status

None